linear regression with hc1 robust standard errors Search Results


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DSMZ laccase producers isolate hc1
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Sangon Biotech dna fragment containing the t7 promoter, t7 terminator, hdv ribozyme, hc1 and hc2 sequences
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Dna Fragment Containing The T7 Promoter, T7 Terminator, Hdv Ribozyme, Hc1 And Hc2 Sequences, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fluka Chemical phosphoric acid
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Phosphoric Acid, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore lsoproterenol-hc1
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Lsoproterenol Hc1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA hc1
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Hc1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA hc1 (emerck)
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Hc1 (Emerck), supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore 6 m hc1
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
6 M Hc1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Difco trypticase soy broth
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Trypticase Soy Broth, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA analytical hc1
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Analytical Hc1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biochemed Pharmacologicals hc1-6 samples
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
Hc1 6 Samples, supplied by Biochemed Pharmacologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sony video camera sony hdr-hc1
Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), <t>HC1</t> (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .
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Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), HC1 (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .

Journal: Frontiers in Veterinary Science

Article Title: Rapid Development of an Effective Newcastle Disease Virus Vaccine Candidate by Attenuation of a Genotype VII Velogenic Isolate Using a Simple Infectious Cloning System

doi: 10.3389/fvets.2020.00648

Figure Lengend Snippet: Genome organization of NDH3 and the schematic strategy for construction of the full-length pBR322-NHD3 and mutant pBR322-NHD3-mF constructs. (A) Ten overlapping PCR fragments covering the whole DHN3 genome are indicated by N, MIMI, PD, PD1, PD2, PD3, L1, L2, L3, and L4, respectively. The three subgenome-sized plasmids, pBR322-PNP, pBR322-PDP, and pBR322-LPD3, were constructed based on the indicated restriction sites and used for the construction of pBR322-DHN3. (B) The pBR322-Base plasmid was constructed by inserting a synthetic DNA fragment into pBR322 between Not I and Nhe I site. The DNA fragment is composed of the T7 terminator, HDV ribozyme, HC2 (5′ end from 1-141nt of DHN3), HC1 (3′ end from 15192-15159nt of DHN3) and the T7 promoter sequence. The PNP, PDP and LPD3 fragments excised from the three subgenome-sized plasmids were ligated, and then recombined with pBR322-Base to produce the full-length pBR322-DHN3. (C) pBR322-DHN3-mF was constructed by recombination of three fragments. The large fragment prepared from SmaI- digestion pBR322-DHN3, and the two smaller fragments containing the mutated sequence marked in red were generated by PCR amplification with two pairs of specially designed primers .

Article Snippet: Construction of pBR322-Base: a DNA fragment containing the T7 promoter, T7 terminator, HDV (Hepatitis delta virus) Ribozyme, HC1 and HC2 sequences was synthesized from Sangon Biotech (Shanghai) Co (Sangon Biotech), and inserted into pBR322 vector between Not I and Nhe I sites.

Techniques: Mutagenesis, Construct, Plasmid Preparation, Sequencing, Generated, Amplification